What you'll learn
Perform gene expression analysis
Create a Protein-protein interaction map and calculate hub genes using Cytoscape
Screening of potential drug targets
ADMET analysis of drugs DruLito and OSIRIS
Perform molecular docking using Autodock Vina
Visualization of the docked complex using Biovia Discovery Studio
Enrichment analysis using EnrichR
Finding microRNAs associated with hub genes using miRNet
Manuscript preparation and using a bibliographic management tool (Zotero will be used)
Select a proper journal (Scopus indexed as well as PubMed indexed) for your first paper.
Uploading a paper to BioRxiv (getting DOI for your first paper)
Requirements
No prior knowledge of softwares for drug discovery is required as in this course no coding has been used
This course is meant for all the those who have love for life sciences and want to start their research career
This course is meant for all those who are interested in learning computer aided drug designing
This course is meant for those who are interested in learning molecular docking
This course is meant for those who are interested in bioinformatics
This course is meant for all those who are interested in learning bioinformatics (project based approach)
Description
A complete guide to the development of ideas for in-silico research to the publication of a paper - yes this is what you getFrom idea development for research to writing and publishing a paper, yes that is what you get. Learn the basics of data analysis for gene expression analysis, molecular docking, visualization of the docked complex, enrichment analysis, microRNA identification production of publication-quality images, writing a research article properly, and using Zotero - a free reference management tool. In this course, you will be getting a proper idea of how can a set of genes can be used to design drugs and how using molecular docking techniques we can validate our results for the best drug. You will be getting a good idea of 'drug repurposing' also after the completion of the hands-on training session associated with this course. No prior knowledge or skills are required to attain this course and this course is deliberately kept straightforward for new learners. In this course, you will get all the necessary skills required to write a research article on your own, guidelines for selecting a proper journal for your manuscript, and also you can extend these research ideas to wet-lab experiments as well.ALL THE BEST:)
Overview
Section 1: Introduction
Lecture 1 Intro to the course
Section 2: Study Plan
Lecture 2 Study Plan
Section 3: Data analysis and Manipulation
Lecture 3 Procuring Data
Lecture 4 Data Manipulation
Section 4: Finding the Differentially Expressed Genes (DEGs)
Lecture 5 Creating Venn diagram
Section 5: Identifying Hub genes from Protein-protein interaction network
Lecture 6 Creating PPI and using STRING tool and identifying Hub genes using Cytoscape
Section 6: Identifying drugs associated with hub genes
Lecture 7 Identification of drugs using Drug gene interaction database (DGIdb)
Section 7: Screening of drugs
Lecture 8 Using DruLiTo and OSIRIS for Screening of Drugs
Section 8: Molecular Docking study
Lecture 9 Downloading PDB files of proteins
Lecture 10 UCSF Chimera and AutoDock Vina Installation
Lecture 11 Energy minimization of protein and preparing the protein for molecular docking
Lecture 12 Energy minimization of ligand and preparing it for docking
Lecture 13 Finding pockets for our protein of interest
Lecture 14 Molecular Docking study
Lecture 15 Creating the docked complex file
Lecture 16 Visualization of the docked complex
Section 9: Producing publication quality images
Lecture 17 Using Adobe Photoshop for producing images for publication
Lecture 18 Using Adobe Photoshop for Producing publication quality images
Section 10: Enrichment analysis
Lecture 19 Enrichment analysis using Enrichr
Section 11: Identification of microRNAs associated with top ten hub genes
Lecture 20 Using miRNet for identification of microRNAs associated with top ten hub genes
Section 12: Writing your first paper
Lecture 21 Anatomy of a research article and selection of correct journal
Lecture 22 Preparing manuscript for journal and using Zotero for reference management
Lecture 23 Uploading your manuscript to BioRxiv
Section 13: Course Conclusion
Lecture 24 Course Conclusion
Anyone interested in any branch of life science,Aspiring researchers,Anyone who wants to know how a drug discovery process looks like,Anyone interested in in-silico research,Anyone who want to learn computer aided drug designing,Anyone interested in learning molecular docking,Anyone interested in bioinformatics


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